《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2024年06 页码: 64-70 栏目: 出版日期: 2024-06-10

Title:Optimization of Liquid Fermentation and Polysaccharide Extraction of Sanghuangporus sanghuang

文章编号:11

作者:李德毓1; 2; 司豆豆1; 2; 彭浩1; 2; 曹伟刚3; 郑晓芹3; 张升明4（1.陕西理工大学 生物科学与工程学院，陕西 汉中723001； 2.陕西省汉中市科技资源两全中心，陕西 汉中 723001； 3.陕西省汉中市秦巴生态；ぶ行，陕西 汉中 723001； 4.陕西省西乡县食用菌钻研所，陕西 汉中 723500）

Author(s):LI Deyu1; 2;  SI Doudou1; 2 ;  PENG Hao1; 2;  CAO Weigang3;  ZHENG Xiaoqin3;  ZHANG Shengming4(1.School of Biolgical Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China; 2. Shaanxi Hanzhong City Science and Technology Resources Coordination Center, Hanzhong 723001, China; 3. Qinba Ecological Protection Center, Hanzhon

关键词:桑树；; 液体发酵; 多糖; 超声波辅助提取法

Keywords:Sanghuangporus sanghuang;  liquid fermentation;  polysaccharide;  ultrasonic extraction

DOI:10.11942/j.issn1002-2767.2024.06.0064

文件标志码:A

提要:桑树；剖且恢钟涤杏帕家┬У囊┯枚嗫拙，其多糖是重要活性成分之一，拥有抗肿瘤、抗氧化、免疫调节和其他药理作用。为提高桑树；凭刻甯沙良吧；贫嗵堑寐，选取单成分和正交试验对影响；凭刻甯沙恋脑炀突浞郊耙禾宸⒔驮炀颓疤峤杏呕；利用超声波辅助提取法对；贫嗵翘崛」ひ涨疤峤杏呕。了局批注，桑树；谱罴言炀突浞轿砹迨恚ㄈテぃ200 g·L-1、乳糖32 g·L-1，胰蛋白胨10 g·L-1，NaCl 2.00 g·L-1，MgSO4·7H2O 1 g·L-1，VB1 10.0 mg·L-1，获得菌丝体干沉为2.82 g·L-1。在温度28 ℃，接种量9%，转速180 r·min-1，pH6.0前提下，最大菌丝量为3.12 g·L-1。；贫嗵亲罴烟崛」ひ瘴üβ200 W，料液比1∶60 g·mL-1，提取功夫40 min，温度50 ℃，此时；贫嗵堑寐饰5.54%。注明本钻研优化的桑树；圃炀突浞健⒎⒔颓疤峒岸嗵翘崛」ひ蘸侠怼⒖尚。

Abstract:Sanghuangporus sanghuang is a medicinal porous bacterium with good medicinal efficacy, and its polysaccharide is one of the main active ingredients, with antitumor, antioxidant, immunomodulatory and other pharmacological effects. In order to improve the dry weight of S. sanghuang mycelia and the yield of S.sanghuang polysaccharide, single factor and orthogonal test were used to optimize the medium formula and liquid fermentation culture conditions affecting the dry weight of S.sanghuang mycelia. Ultrasonic assisted extraction method was used to optimize the extraction conditions of polysaccharide yield of S.sanghuang. The results showed that the optimal medium formula of S. sanghuang was potatoes (peeled) 200 g·L-1,lactose 32 g·L-1, tryptone 10 g·L-1, NaCl 2.00 g·L-1, MgSO4·7H2O 1 g·L-1, VB1 10.0 mg·L-1, and mycelium dry weight was 2.82 g·L-1. Under the conditions of temperature 28 ℃, inoculation amount 9%, rotational speed 180 r·min-1 and pH6, the maximum mycelium amount was 3.12 g·L-1. The optimal extraction process was as follows: ultrasonic power 200 W, solid-liquid ratio 1∶60, extraction time 40 min, temperature 50 ℃, the yield of polysaccharides was 5.54%. The results showed that the optimized culture medium formula, fermentation conditions and polysaccharide extraction technology of S. sanghuang were reasonable and feasible, which could provide reference for liquid fermentation of S. sanghuang mycelium to produce polysaccharide.